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u6 expression vectors  (Addgene inc)


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    Addgene inc u6 expression vectors
    U6 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+vectors/SpCas9_sgRNA_expression_in_pBluescript+(Plasmid+%23122089)/pmc12957561-33-23-26
    Average 93 stars, based on 12 article reviews
    u6 expression vectors - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency
    Article Snippet: To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences and 3’ extension sequences (RTT, PBS & pseudoknot) were amplified with indicated primers (Supplementary table) using Phusion master mix (ThermoFisher Scientific, #F-548L). .. These amplicons were subsequently cloned into the sgRNA, pegRNA or epegRNA U6 expression vectors (Addgene, #122089) by the Gibson assembly method (NEB, #E2611L). .. To generate sgRNA expression plasmids, annealed oligos were cloned into BfuAI-digested vectors.

    Article Title: Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
    Article Snippet: .. The PCR product was cloned into the EcoRI and BamHI sites of the pBluescript K(+) shuttle vector (Addgene). ..

    Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo
    Article Snippet: .. Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold sequence (for sgRNAs) or a 3′ direct repeat (DR)-spacer sequence (for crRNAs) were cloned into U6 expression vectors (Addgene, #122089 and #217005) that had been linearized by BstXI and BlpI digestion, using the Gibson assembly method (New England Biolabs [NEB], #E2611L). ..

    Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
    Article Snippet: .. 86 MATERIALS AND METHODS 87 General methods and molecular cloning 88 Expression plasmids used for sgRNAs or crRNAs were constructed based on previously 89 described backbones.35 Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold 90 sequence (for sgRNAs) or a 3’ Direct Repeat (DR)-spacer sequence (for crRNAs) were cloned 91 into U6 expression vectors (Addgene ##122089 and ##217005) that had been linearized by BstXI 92 and BlpI digestion, using the Gibson Assembly method (NEB, #E2611L). ..

    Article Title: Sexual dimorphic distribution of cannabinoid 1 receptor mRNA in adult C57BL/6J mice.
    Article Snippet: Shenzhen Key Lab of Neuropsychiatric Modulation, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Key Laboratory of Brain Connectome and Manipulation, the Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, China University of Chinese Academy of Sciences, Beijing, China

    Expressing:

    Article Title: Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency
    Article Snippet: To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences and 3’ extension sequences (RTT, PBS & pseudoknot) were amplified with indicated primers (Supplementary table) using Phusion master mix (ThermoFisher Scientific, #F-548L). .. These amplicons were subsequently cloned into the sgRNA, pegRNA or epegRNA U6 expression vectors (Addgene, #122089) by the Gibson assembly method (NEB, #E2611L). .. To generate sgRNA expression plasmids, annealed oligos were cloned into BfuAI-digested vectors.

    Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo
    Article Snippet: .. Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold sequence (for sgRNAs) or a 3′ direct repeat (DR)-spacer sequence (for crRNAs) were cloned into U6 expression vectors (Addgene, #122089 and #217005) that had been linearized by BstXI and BlpI digestion, using the Gibson assembly method (New England Biolabs [NEB], #E2611L). ..

    Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
    Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..

    Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
    Article Snippet: .. 86 MATERIALS AND METHODS 87 General methods and molecular cloning 88 Expression plasmids used for sgRNAs or crRNAs were constructed based on previously 89 described backbones.35 Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold 90 sequence (for sgRNAs) or a 3’ Direct Repeat (DR)-spacer sequence (for crRNAs) were cloned 91 into U6 expression vectors (Addgene ##122089 and ##217005) that had been linearized by BstXI 92 and BlpI digestion, using the Gibson Assembly method (NEB, #E2611L). ..

    Polymerase Chain Reaction:

    Article Title: Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
    Article Snippet: .. The PCR product was cloned into the EcoRI and BamHI sites of the pBluescript K(+) shuttle vector (Addgene). ..

    Plasmid Preparation:

    Article Title: Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
    Article Snippet: .. The PCR product was cloned into the EcoRI and BamHI sites of the pBluescript K(+) shuttle vector (Addgene). ..

    Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
    Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..

    Sequencing:

    Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo
    Article Snippet: .. Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold sequence (for sgRNAs) or a 3′ direct repeat (DR)-spacer sequence (for crRNAs) were cloned into U6 expression vectors (Addgene, #122089 and #217005) that had been linearized by BstXI and BlpI digestion, using the Gibson assembly method (New England Biolabs [NEB], #E2611L). ..

    Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
    Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..

    Article Title: Pore-Forming Cardiotoxin VVA2 (Volvatoxin A2) Variant I82E/L86K Is an Atypical Duplex-Specific Nuclease
    Article Snippet: .. Sequence analysis of the fragments was conducted by NCBI blastn program (access date: 16 February 2022) with sequences of pUC19/pET28a and pBluescript II SK (+) vectors from Addgene (Watertown, MA, USA). ..

    Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
    Article Snippet: .. 86 MATERIALS AND METHODS 87 General methods and molecular cloning 88 Expression plasmids used for sgRNAs or crRNAs were constructed based on previously 89 described backbones.35 Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold 90 sequence (for sgRNAs) or a 3’ Direct Repeat (DR)-spacer sequence (for crRNAs) were cloned 91 into U6 expression vectors (Addgene ##122089 and ##217005) that had been linearized by BstXI 92 and BlpI digestion, using the Gibson Assembly method (NEB, #E2611L). ..

    Article Title: Sexual dimorphic distribution of cannabinoid 1 receptor mRNA in adult C57BL/6J mice.
    Article Snippet: Shenzhen Key Lab of Neuropsychiatric Modulation, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Key Laboratory of Brain Connectome and Manipulation, the Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, China University of Chinese Academy of Sciences, Beijing, China

    Construct:

    Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
    Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..

    Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
    Article Snippet: .. 86 MATERIALS AND METHODS 87 General methods and molecular cloning 88 Expression plasmids used for sgRNAs or crRNAs were constructed based on previously 89 described backbones.35 Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold 90 sequence (for sgRNAs) or a 3’ Direct Repeat (DR)-spacer sequence (for crRNAs) were cloned 91 into U6 expression vectors (Addgene ##122089 and ##217005) that had been linearized by BstXI 92 and BlpI digestion, using the Gibson Assembly method (NEB, #E2611L). ..

    Marker:

    Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
    Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..

    Derivative Assay:

    Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
    Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..

    Amplification:

    Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
    Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..

    Activation Assay:

    Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
    Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..

    Molecular Cloning:

    Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
    Article Snippet: .. 86 MATERIALS AND METHODS 87 General methods and molecular cloning 88 Expression plasmids used for sgRNAs or crRNAs were constructed based on previously 89 described backbones.35 Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold 90 sequence (for sgRNAs) or a 3’ Direct Repeat (DR)-spacer sequence (for crRNAs) were cloned 91 into U6 expression vectors (Addgene ##122089 and ##217005) that had been linearized by BstXI 92 and BlpI digestion, using the Gibson Assembly method (NEB, #E2611L). ..

    Cloning:

    Article Title: Sexual dimorphic distribution of cannabinoid 1 receptor mRNA in adult C57BL/6J mice.
    Article Snippet: Shenzhen Key Lab of Neuropsychiatric Modulation, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Key Laboratory of Brain Connectome and Manipulation, the Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, China University of Chinese Academy of Sciences, Beijing, China



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    Addgene inc pbluescript sk ii vector

    Pbluescript Sk Ii Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+vectors/pBluescript+SK+%2B%2F-+mTRF1+(Plasmid+%2312303)/pmc12378376-105-30-33
    Average 93 stars, based on 1 article reviews
    pbluescript sk ii vector - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    pbluescript vector  (New England Biolabs)
    99
    New England Biolabs pbluescript vector

    Pbluescript Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+vectors/XbaI/pmc12228689-243-36-41
    Average 99 stars, based on 1 article reviews
    pbluescript vector - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

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    Journal: bioRxiv

    Article Title: CANVAS causing AAGGG repeat expansions cause tissue-specific reduction in RFC1 expression and increase sensitivity to DNA damage

    doi: 10.1101/2025.11.18.688292

    Figure Lengend Snippet:

    Article Snippet: Briefly, Firefly Luciferase plasmid and the repeat containing pBluescript vectors were digested with XbaI and NotI restriction enzymes (NEB), while pGint plasmid and pBluescript vectors were digested with NotI and BamHI restriction enzymes (NEB) at 37°C for 1 hour.

    Techniques: Luciferase